Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thioredoxin reductase (TRXR) Standard Curve.)
Rat Thioredoxin Reductase (TRXR) ELISA Kit | TRXR elisa kit
Rat Thioredoxin Reductase (TRXR) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thioredoxin reductase (TRXR) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thioredoxin reductase (TRXR) Standard Curve.)
Background: Thioredoxin reductase 1, cytoplasmic is a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. [provided by RefSeq, Jun 2017]
Uniprot Description
TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.
Protein type: Oxidoreductase; EC 1.8.1.9; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine
Cellular Component: nucleoplasm; cell soma; mitochondrion; cytoplasm; nucleolus; nucleus; cytosol
Molecular Function: selenate reductase activity; protein homodimerization activity; thioredoxin-disulfide reductase activity; mercury (II) reductase activity; FAD binding; NAD(P)H oxidase activity; mercury ion binding; NADP binding
Biological Process: response to drug; benzene and derivative metabolic process; gastrulation; selenocysteine metabolic process; cell proliferation; response to selenium ion; response to hyperoxia; glutathione metabolic process; mesoderm formation; cell redox homeostasis; hydrogen peroxide catabolic process; detoxification of mercury ion; methylmercury metabolic process; response to oxidative stress; response to axon injury; protein tetramerization; placenta development
Research Articles on TRXR
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Product Notes
The Rat TRXR txnrd1 (Catalog #AAA9712689) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712689 ELISA Kit recognizes Rat TRXR. It is sometimes possible for the material contained within the vial of "Thioredoxin Reductase (TRXR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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