Typical Testing Data/Standard Curve (for reference only)
Rat TDGF1 ELISA Kit | TDGF1 elisa kit
Rat TDGF1 (Teratocarcinoma Derived Growth Factor 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TDGF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TDGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TDGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TDGF1. You can calculate the concentration of Rat TDGF1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Uniprot Description
TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.
Protein type: Membrane protein, peripheral; Motility/polarity/chemotaxis; Cell adhesion; Cell cycle regulation; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: extrinsic to plasma membrane; Golgi apparatus; extracellular space; cell surface; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; nucleus; lipid raft
Molecular Function: protein binding; growth factor activity; receptor binding
Biological Process: somatic stem cell maintenance; activation of MAPK activity; heart development; regulation of signal transduction; gastrulation; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; positive regulation of fibroblast proliferation; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; vasculogenesis; positive regulation of cell-matrix adhesion; cell differentiation; epidermal growth factor receptor signaling pathway; in utero embryonic development; morphogenesis of a branching structure; embryonic development; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; negative regulation of apoptosis
Research Articles on TDGF1
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Product Notes
The Rat TDGF1 tdgf1 (Catalog #AAA2515562) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515562 ELISA Kit recognizes Rat TDGF1. It is sometimes possible for the material contained within the vial of "TDGF1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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