Rat Taurine Transporter (TAUT) ELISA Kit | TAUT elisa kit

Rat Taurine Transporter (TAUT) ELISA Kit

Cat. Num. AAA2000221

• Gene Names: SLC6A6; TAUT
• Reactivity: Rat
• Synonyms: Taurine Transporter (TAUT); Rat Taurine Transporter (TAUT) ELISA Kit; SLC6A6; Sodium And Chloride Dependent Taurine Transporter; Solute Carrier Family 6 Member 6; Neurotransmitter Transporter; Taurine; TAUT elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Taurine Transporter (TAUT).; No significant cross-reactivity or interference between Taurine Transporter (TAUT) and analogues was observed.
• Sequence Length: 620

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.052ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Taurine Transporter (TAUT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Taurine Transporter (TAUT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAUT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Taurine Transporter (TAUT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Taurine Transporter (TAUT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Taurine Transporter (TAUT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Taurine Transporter (TAUT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TAUT elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 799339
• NCBI GeneID: 6533
• UniProt Accession #: P31641; Q9BRI2; Q9BXB0; B2RNU7; AAB25509
• Molecular Weight: 22,744 Da
• NCBI Official Full Name: taurine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 6
• NCBI Official Symbol: SLC6A6
• NCBI Official Synonym Symbols: TAUT
• NCBI Protein Information: sodium- and chloride-dependent taurine transporter
• UniProt Protein Name: Sodium- and chloride-dependent taurine transporter
• UniProt Gene Name: SLC6A6
• UniProt Entry Name: SC6A6_HUMAN

NCBI Description

This gene encodes a multi-pass membrane protein that is a member of a family of sodium and chloride-ion dependent transporters. The encoded protein transports taurine and beta-alanine. There is a pseudogene for this gene on chromosome 21. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

TAUT: Required for the uptake of taurine. Transports both taurine and beta-alanine which requires sodium ions. Chloride ions are necessary for optimal uptake. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A6 subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: taurine binding; neurotransmitter:sodium symporter activity; taurine:sodium symporter activity

Biological Process: amino acid metabolic process; taurine transport; neurotransmitter transport; amino acid transport; transport; ion transport; transmembrane transport