Rat SLC ELISA Kit | SLC elisa kit
Rat SLC (Secondary Lymphoid Tissue Chemokine) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SLC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat SLC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat SLC. You can calculate the concentration of Rat SLC in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014]
Uniprot Description
CCL21: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9p13
Cellular Component: extracellular space; extracellular region
Molecular Function: chemokine activity; chemokine receptor binding; CCR7 chemokine receptor binding
Biological Process: positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; formation of immunological synapse; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of cell adhesion mediated by integrin; positive regulation of JNK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; positive regulation of pseudopodium formation; cell-cell signaling; positive regulation of actin filament polymerization; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; T cell costimulation; ruffle organization and biogenesis; dendritic cell chemotaxis; establishment of T cell polarity; immune response; positive regulation of cell-matrix adhesion; inflammatory response
