Rat SHBG ELISA Kit | SHBG elisa kit

Rat SHBG (Sex Hormone-Binding Globulin) ELISA Kit

Cat. Num. AAA2502327

• Gene Names: SHBG; ABP; SBP; TEBG
• Reactivity: Rat
• Synonyms: SHBG; Rat SHBG (Sex Hormone-Binding Globulin) ELISA Kit; SHBG elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes Rat SHBG in samples. No significant cross-reactivity or interference between Rat SHBG and analogues was observed.
• Sequence Length: 402

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.63-1000pg/mL
• Sensitivity: 9.38pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat SHBG were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat SHBG were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SHBG elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat SHBG concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SHBG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat SHBG. You can calculate the concentration of Rat SHBG in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7382460
• NCBI GeneID: 6462
• NCBI Accession #: NP_001031.2
• NCBI GenBank Nucleotide #: NM_001040.4
• UniProt Accession #: P04278; P14689; Q16616; Q3MIL0; Q6ISD2; B0FWH4; E9PGW1; F5H5Z8; I3L1N7
• Molecular Weight: 41,712 Da
• NCBI Official Full Name: sex hormone-binding globulin isoform 1
• NCBI Official Synonym Full Names: sex hormone-binding globulin
• NCBI Official Symbol: SHBG
• NCBI Official Synonym Symbols: ABP; SBP; TEBG
• NCBI Protein Information: sex hormone-binding globulin; sex steroid-binding protein; testis-specific androgen-binding protein; testosterone-binding beta-globulin; testosterone-estradiol-binding globulin; testosterone-estrogen-binding globulin
• UniProt Protein Name: Sex hormone-binding globulin
• Protein Family: Sex hormone-binding globulin
• UniProt Gene Name: SHBG
• UniProt Synonym Gene Names: SHBG; SBP; ABP; TeBG
• UniProt Entry Name: SHBG_HUMAN

NCBI Description

This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: extracellular region

Molecular Function: androgen binding

Biological Process: primary spermatocyte growth