Typical Standard Curve/Testing Data
Rat Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit | ARHGEF7 elisa kit
Rat Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit
Gene Names
Arhgef7; P85spr; Pak3bp
Reactivity
Rat
Synonyms
Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7); Rat Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit; PAK3; BETA-PIX; COOL1; P50; P50BP; P85; P85SPR; PIXB; SH3 Domain-Containing Proline-Rich Protein; PAK-Interacting Exchange Factor Beta; ARHGEF7 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of ARHGEF7. No significant cross-reactivity or interference between ARHGEF7 and analogues was observed.
Sequence Length
646
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.064ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ARHGEF7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ARHGEF7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for ARHGEF7 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ARHGEF7 in rat tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ARHGEF7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ARHGEF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ARHGEF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ARHGEF7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ARHGEF7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ARHGEF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ARHGEF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ARHGEF7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,140 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 7 isoform b
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 7
NCBI Official Symbol
Arhgef7
NCBI Official Synonym Symbols
P85spr; Pak3bp
NCBI Protein Information
rho guanine nucleotide exchange factor 7
UniProt Protein Name
Rho guanine nucleotide exchange factor 7
Protein Family
UniProt Gene Name
Arhgef7
UniProt Synonym Gene Names
Pak3bp; Pixb
NCBI Description
Rac/Cdc42-specific guanine nucleotide exchange factor; mediates bFGF-induced neurite outgrowth [RGD, Feb 2006]
Uniprot Description
Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons ().
Research Articles on ARHGEF7
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Product Notes
The Rat ARHGEF7 arhgef7 (Catalog #AAA453914) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA453914 ELISA Kit recognizes Rat ARHGEF7. It is sometimes possible for the material contained within the vial of "Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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