Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Regenerating islet-derived protein 3-alpha (REG3A) Standard Curve.)
Regenerating Islet-Derived Protein 3-alpha (REG3A) ELISA Kit | REG3A elisa kit
Rat Regenerating Islet-Derived Protein 3-alpha (REG3A) ELISA Kit
Gene Names
Reg3a; Pap2; PapII
Reactivity
Rat
Synonyms
Regenerating Islet-Derived Protein 3-alpha (REG3A); Rat Regenerating Islet-Derived Protein 3-alpha (REG3A) ELISA Kit; REG3A; HIP; PAP; PAP-H; PAP1; PBCGF; REG-III; REG3; PAP homologous protein; hepatocarcinoma-intestine-pancreas; pancreatic beta cell growth factor; pancreatitis-associated protein; proliferation-inducing prote; REG3A elisa kit
Reactivity
Rat
Specificity
Rat Regenerating islet-derived protein 3-alpha (REG3A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat REG3A. No significant cross-reactivity or interference between Rat REG3A and analogues was observed.
Sequence Length
174
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Regenerating islet-derived protein 3-alpha (REG3A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Regenerating islet-derived protein 3-alpha (REG3A) Standard Curve.)
Related Product Information for REG3A elisa kit
Description: This Rat Regenerating islet-derived protein 3-alpha (REG3A) ELISA Kit employs a two-site sandwich ELISA to quantitate REG3A in samples. An antibody specific for REG3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Regenerating islet-derived protein 3 alpha is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The predicted protein had the same size as the rat protein and showed 71% amino acid identity, the 6 half-cysteines being in identical positions. As in the rat, the protein was expressed at very low levels in control tissues and overexpressed during the acute phase of pancreatitis in humans. The protein could be immunolocalized to the apical regions of acinar cells. The protein was also quantifiable in serum with an elevation of levels in pancreatitis, suggesting its possible use as a biologic marker of the disease.
Background: Regenerating islet-derived protein 3 alpha is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The predicted protein had the same size as the rat protein and showed 71% amino acid identity, the 6 half-cysteines being in identical positions. As in the rat, the protein was expressed at very low levels in control tissues and overexpressed during the acute phase of pancreatitis in humans. The protein could be immunolocalized to the apical regions of acinar cells. The protein was also quantifiable in serum with an elevation of levels in pancreatitis, suggesting its possible use as a biologic marker of the disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
regenerating islet-derived protein 3-alpha
NCBI Official Synonym Full Names
regenerating islet-derived 3 alpha
NCBI Official Symbol
Reg3a
NCBI Official Synonym Symbols
Pap2; PapII
NCBI Protein Information
regenerating islet-derived protein 3-alpha
UniProt Protein Name
Regenerating islet-derived protein 3-alpha
Protein Family
UniProt Gene Name
Reg3a
UniProt Synonym Gene Names
Pap2; Reg3; REG-3-alpha; REG 3; Reg III-alpha
NCBI Description
lectin-related secretory protein; overexpressed during the acute phase of pancreatitis [RGD, Feb 2006]
Uniprot Description
Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway ().
Research Articles on REG3A
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Product Notes
The Rat REG3A reg3a (Catalog #AAA9711928) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711928 ELISA Kit recognizes Rat REG3A. It is sometimes possible for the material contained within the vial of "Regenerating Islet-Derived Protein 3-alpha (REG3A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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