Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit | PDK1 elisa kit

Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit

Cat. Num. AAA2704633

• Gene Names: PDK2; PDHK2; PDKII
• Reactivity: Rat
• Synonyms: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1); Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1; mitochondrial; PDK1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1).; No significant cross-reactivity or interference between Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) and analogues was observed.
• Sequence Length: 407

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.58ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDK1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19923736
• NCBI GeneID: 5164
• NCBI Accession #: NP_002602.2
• NCBI GenBank Nucleotide #: NM_002611.4
• UniProt Accession #: Q15119; Q6P515; Q9BS05; A8K3A7; B3KNW0
• Molecular Weight: 38,864 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 2 isoform 1
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase 2
• NCBI Official Symbol: PDK2
• NCBI Official Synonym Symbols: PDHK2; PDKII
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 2
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: PDK2
• UniProt Synonym Gene Names: PDHK2; PDH kinase 2; PDKII

NCBI Description

This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: ATYPICAL group; EC 2.7.11.2; Kinase, protein; Mitochondrial; PDHK family; PDHK subfamily; Protein kinase, atypical

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: cytosol; mitochondrial matrix; mitochondrial pyruvate dehydrogenase complex; mitochondrion; nucleoplasm

Molecular Function: ATP binding; protein homodimerization activity; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity

Biological Process: cellular response to nutrient; cellular response to reactive oxygen species; glucose homeostasis; glucose metabolic process; insulin receptor signaling pathway; intrinsic apoptotic signaling pathway by p53 class mediator; protein amino acid phosphorylation; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of cellular ketone metabolic process; regulation of gluconeogenesis; regulation of glucose metabolic process; regulation of pH