Rat PR domain zinc finger protein 16 (PRDM16) ELISA Kit | PRDM16 elisa kit

Rat PR domain zinc finger protein 16 (PRDM16) ELISA Kit

Cat. Num. AAA2801936

• Reactivity: Rat
• Synonyms: PR domain zinc finger protein 16 (PRDM16); Rat PR domain zinc finger protein 16 (PRDM16) ELISA Kit; KIAA1675; MEL1; MGC166915; PFM13; MDS1/EVI1-like; PR-domain zinc finger protein 16; transcription factor MEL1; PRDM16 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat PRDM16. No significant cross-reactivity or interference between Rat PRDM16 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate PRDM16 in samples. An antibody specific for PRDM16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDM16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

Related Product Information for PRDM16 elisa kit

The 1,257-amino acid MEL1 protein shares 56% amino acid identity with MDS1/EVI1 and has a similar domain structure. The first 222 amino acids of MEL1 are highly homologous to the PR domain of MDS1, and the rest of the MEL1 sequence is homologous to EVI1. Following the N-terminal PR domain, MEL1 has a zinc finger DNA-binding domain, a proline-rich domain, a repressor domain, a second zinc finger DNA-binding domain, and a C-terminal acidic domain. Northern blot and RT-PCR analyses of various leukemia cell lines revealed a major 8-kb MEL1 transcript only in cells with t(1;3). In human tissues, RT-PCR showed MEL1 expression only in uterus and fetal kidney. MEL1 and MEL1S had apparent molecular masses of 170 and 150 kD, respectively, and t(1;3)-positive leukemias expressed predominantly MEL1S protein.