Typical Testing Data/Standard Curve (for reference only)
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Rat Platelet Derived Growth Factor Receptor Beta (PDGFRb) ELISA Kit | PDGFRb elisa kit
Rat Platelet Derived Growth Factor Receptor Beta (PDGFRb) ELISA Kit
Gene Names
Pdgfrb; PDGFR-1
Reactivity
Rat
Synonyms
Platelet Derived Growth Factor Receptor Beta (PDGFRb); Rat Platelet Derived Growth Factor Receptor Beta (PDGFRb) ELISA Kit; CD140b; JTK12; PDGF-R-Beta; PDGFR; PDGFR1; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; Beta-type platelet-derived growth factor receptor; PDGFRb elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of PDGFRb. No significant cross-reactivity or interference between PDGFRb and analogues was observed.
Sequence Length
1097
Samples
Rat tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDGFRb were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDGFRb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for PDGFRb elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDGFRb in rat tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PDGFRb. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PDGFRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGFRb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDGFRb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PDGFRb. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PDGFRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGFRb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDGFRb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,828 Da
NCBI Official Full Name
platelet-derived growth factor receptor beta
NCBI Official Synonym Full Names
platelet derived growth factor receptor beta
NCBI Official Symbol
Pdgfrb
NCBI Official Synonym Symbols
PDGFR-1
NCBI Protein Information
platelet-derived growth factor receptor beta
UniProt Protein Name
Platelet-derived growth factor receptor beta
Protein Family
UniProt Gene Name
Pdgfrb
UniProt Synonym Gene Names
Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1
NCBI Description
binds platelet derived growth factor BB homodimer [RGD, Feb 2006]
Uniprot Description
PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent.
Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group
Research Articles on PDGFRb
Similar Products
Product Notes
The Rat PDGFRb pdgfrb (Catalog #AAA2704138) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2704138 ELISA Kit recognizes Rat PDGFRb. It is sometimes possible for the material contained within the vial of "Platelet Derived Growth Factor Receptor Beta (PDGFRb), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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