Typical Testing Data/Standard Curve (for reference only)
Rat Pancreatic Alpha-amylase ELISA Kit | AMY2A elisa kit
Rat Pancreatic Alpha-amylase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat OLR1/LOX1 antibody. OLR1/LOX1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat OLR1/LOX1 Antibody is added and binds to OLR1/LOX1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OLR1/LOX1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat OLR1/LOX1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015]
Uniprot Description
AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]
Protein type: EC 3.2.1.1; Secreted; Hydrolase; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose
Chromosomal Location of Human Ortholog: 1p21
Cellular Component: extracellular space; extracellular region
Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding
Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis
Research Articles on AMY2A
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Product Notes
The Rat AMY2A amy2a (Catalog #AAA1603447) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603447 ELISA Kit recognizes Rat AMY2A. It is sometimes possible for the material contained within the vial of "Pancreatic Alpha-amylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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