Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Palmitoyl-protein thioesterase 1 (PPT1) Standard Curve.)
Rat Palmitoyl-Protein Thioesterase 1 (PPT1) ELISA Kit | PPT1 elisa kit
Rat Palmitoyl-Protein Thioesterase 1 (PPT1) ELISA Kit
Gene Names
Ppt1; Ppt
Reactivity
Rat
Synonyms
Palmitoyl-Protein Thioesterase 1 (PPT1); Rat Palmitoyl-Protein Thioesterase 1 (PPT1) ELISA Kit; PPT1; RP11-115D7.2; CLN1; INCL; PPT; ceroid-palmitoyl-palmitoyl-protein thioesterase 1; palmitoyl-protein hydrolase 1; PPT1 elisa kit
Reactivity
Rat
Specificity
Rat Palmitoyl-protein thioesterase 1 (PPT1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PPT1. No significant cross-reactivity or interference between Rat PPT1 and analogues was observed.
Sequence Length
306
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Palmitoyl-protein thioesterase 1 (PPT1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Palmitoyl-protein thioesterase 1 (PPT1) Standard Curve.)
Related Product Information for PPT1 elisa kit
Description: This Rat Palmitoyl-protein thioesterase 1 (PPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PPT1 in samples. An antibody specific for PPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PPT1 is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. The cDNA predicted a 306-amino acid polypeptide that contains a 25-amino acid signal peptide, 3 N-linked glycosylation sites, and consensus motifs characteristic of thioesterases. Northern blot analysis revealed ubiquitous expression of a single 2.5-kb mRNA, with highest expression in lung, brain, and heart.
Background: PPT1 is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. The cDNA predicted a 306-amino acid polypeptide that contains a 25-amino acid signal peptide, 3 N-linked glycosylation sites, and consensus motifs characteristic of thioesterases. Northern blot analysis revealed ubiquitous expression of a single 2.5-kb mRNA, with highest expression in lung, brain, and heart.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
palmitoyl-protein thioesterase 1
NCBI Official Synonym Full Names
palmitoyl-protein thioesterase 1
NCBI Official Symbol
Ppt1
NCBI Official Synonym Symbols
Ppt
NCBI Protein Information
palmitoyl-protein thioesterase 1
UniProt Protein Name
Palmitoyl-protein thioesterase 1
Protein Family
UniProt Gene Name
Ppt1
UniProt Synonym Gene Names
Ppt; PPT-1
UniProt Entry Name
PPT1_RAT
NCBI Description
deficiency causes drastic neurodegeneration; may protect neurons from excitotoxicity; may mediate synaptic plasticity [RGD, Feb 2006]
Research Articles on PPT1
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Product Notes
The Rat PPT1 ppt1 (Catalog #AAA9712095) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712095 ELISA Kit recognizes Rat PPT1. It is sometimes possible for the material contained within the vial of "Palmitoyl-Protein Thioesterase 1 (PPT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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