Rat Osteopontin (OPN) ELISA Kit | OPN elisa kit

Rat Osteopontin (OPN) ELISA Kit

Cat. Num. AAA9711732

• Gene Names: SPP1; OPN
• Reactivity: Rat
• Synonyms: Osteopontin (OPN); Rat Osteopontin (OPN) ELISA Kit; SPP1; BNSP; BSPI; ETA-1; MGC110940; OPN; SPP1/CALPHA1 fusion; bone sialoprotein I; early T-lymphocyte activation 1; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; s; OPN elisa kit

• Reactivity: Rat
• Specificity: Rat Osteopontin (OPN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SPP1. No significant cross-reactivity or interference between Rat SPP1 and analogues was observed.
• Sequence Length: 314

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Osteopontin (OPN) Standard Curve.)

Related Product Information for OPN elisa kit

Description: This Rat Osteopontin (OPN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPP1 in samples. An antibody specific for SPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Osteopontin (OPN), is a secreted multifunctional glyco-phosphoprotein with roles in bone metabolism, immune regulation, tissue remodeling, cell survival, and tumor progression. Gene structure and chromosomal location identify OPN as a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes BSP,BMP1,DSPP, ENAM and MEPE. Murine OPN issynthesized as a 294 amino acid (aa) precursor protein with a predicted 16 aa signal peptide and a highly unusual mature protein sequence, containing 68 acidic aa and 23 potential Ser/Thr phosphorylation sites. Although the predicted molecular weight of OPN is 31 kDa,phosphorylation and N- and O-glycosylation may allow it to appear as large as 75 kDa. Variability in post-translational modifications can influence the activity of OPN.

NCBI and Uniprot Product Information

• NCBI GI #: 126723455
• NCBI GeneID: 100008982
• NCBI Accession #: NP_001075663.1
• NCBI GenBank Nucleotide #: NP_001075663.1
• UniProt Accession #: P31097
• NCBI Official Full Name: osteopontin
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN
• NCBI Protein Information: osteopontin
• UniProt Protein Name: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: SPP-1

Uniprot Description

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.