Rat Neural cell adhesion molecule ligand 1 (NCAM-L1) ELISA Kit | NCAM-L1 elisa kit

Rat Neural cell adhesion molecule ligand 1 (NCAM-L1) ELISA Kit

Cat. Num. AAA269219

• Gene Names: NCAN; CSPG3
• Reactivity: Rat
• Synonyms: Neural cell adhesion molecule ligand 1 (NCAM-L1); Rat Neural cell adhesion molecule ligand 1 (NCAM-L1) ELISA Kit; NCAM-L1 elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 1321

• Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant NCAM-L1 concentration.
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for NCAM-L1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat NCAM-L1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 118600983
• NCBI GeneID: 1463
• NCBI Accession #: NP_004377.2
• NCBI GenBank Nucleotide #: NM_004386.2
• Molecular Weight: 143,093 Da
• NCBI Official Full Name: neurocan core protein
• NCBI Official Synonym Full Names: neurocan
• NCBI Official Symbol: NCAN
• NCBI Official Synonym Symbols: CSPG3
• NCBI Protein Information: neurocan core protein; neurocan proteoglycan; chondroitin sulfate proteoglycan 3 (neurocan)
• UniProt Protein Name: Neurocan core protein
• UniProt Gene Name: NCAN
• UniProt Synonym Gene Names: CSPG3; NEUR
• UniProt Entry Name: NCAN_HUMAN

NCBI Description

Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM, Jul 2002]

Uniprot Description

CSPG3: May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. Belongs to the aggrecan/versican proteoglycan family.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 19p12

Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: chondroitin sulfate metabolic process; axon guidance; extracellular matrix organization and biogenesis; central nervous system development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; regulation of synapse structural plasticity; pathogenesis; cell adhesion; skeletal development; dermatan sulfate biosynthetic process