Rat N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit | NAGase elisa kit

Rat N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit

Cat. Num. AAA452483

• Gene Names: Mgea5; OGA; Ncoat; O-GLCNACASE
• Reactivity: Rat
• Synonyms: N-Acetyl Beta-D-Glucosaminidase (NAGase); Rat N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit; MGEA5; MEA5; NCOAT; Hexosaminidase C; N-Acetylhexosaminidase; Hyaluronidase; Meningioma-Expressed Antigen 5; Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase; NAGase elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of NAGase. No significant cross-reactivity or interference between NAGase and analogues was observed.
• Sequence Length: 916

• Samples: Urine.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.57ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAGase were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAGase were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NAGase elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NAGase in rat urine.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NAGase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NAGase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NAGase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NAGase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18777747
• NCBI GeneID: 154968
• NCBI Accession #: NP_571979.1
• NCBI GenBank Nucleotide #: NM_131904.1
• UniProt Accession #: Q8VIJ5
• Molecular Weight: 91,993 Da
• NCBI Official Full Name: protein O-GlcNAcase
• NCBI Official Synonym Full Names: meningioma expressed antigen 5 (hyaluronidase)
• NCBI Official Symbol: Mgea5
• NCBI Official Synonym Symbols: OGA; Ncoat; O-GLCNACASE
• NCBI Protein Information: protein O-GlcNAcase
• UniProt Protein Name: Protein O-GlcNAcase
• UniProt Gene Name: Mgea5
• UniProt Synonym Gene Names: Hexc; Mea5; OGA

NCBI Description

plays a role in insulin secretion; may be involved in secretion of other glucose regulated hormones [RGD, Feb 2006]

Uniprot Description

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:8034696). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.