Rat Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) ELISA Kit | MYH8 elisa kit

Rat Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) ELISA Kit

Cat. Num. AAA2705108

• Gene Names: MYH8; DA7; MyHC-pn; gtMHC-F; MyHC-peri
• Reactivity: Rat
• Synonyms: Myosin Heavy Chain 8; Skeletal Muscle; Perinatal (MYH8); Rat Myosin Heavy Chain 8; Perinatal (MYH8) ELISA Kit; MyHC-peri; MyHC-pn; MyHC-perinatal; Myosin heavy chain; skeletal muscle; perinatal; MYH8 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8).; No significant cross-reactivity or interference between Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) and analogues was observed.
• Sequence Length: 1937

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 62.50-4000pg/mL
• Sensitivity: < 24.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 153945790
• NCBI GeneID: 4626
• NCBI Accession #: NP_002463.2
• NCBI GenBank Nucleotide #: NM_002472.2
• UniProt Accession #: P13535; Q14910
• Molecular Weight: 222,763 Da
• NCBI Official Full Name: myosin-8
• NCBI Official Synonym Full Names: myosin heavy chain 8
• NCBI Official Symbol: MYH8
• NCBI Official Synonym Symbols: DA7; MyHC-pn; gtMHC-F; MyHC-peri
• NCBI Protein Information: myosin-8
• UniProt Protein Name: Myosin-8
• Protein Family: Myosin
• UniProt Gene Name: MYH8
• UniProt Synonym Gene Names: MyHC-perinatal

NCBI Description

Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009]

Uniprot Description

MYH8: Muscle contraction. Defects in MYH8 are a cause of Carney complex variant (CACOV). Carney complex is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas. Familial cardiac myxomas are associated with spotty pigmentation of the skin and other phenotypes, including primary pigmented nodular adrenocortical dysplasia, extracardiac (frequently cutaneous) myxomas, schwannomas, and pituitary, thyroid, testicular, bone, ovarian, and breast tumors. Cardiac myxomas do not develop in all patients with the Carney complex, but affected patients have at least two features of the complex or one feature and a clinically significant family history. Defects in MYH8 are a cause of distal arthrogryposis type (DA7). A hereditary distal arthrogryposis characterized by an inability to open the mouth fully (trismus) and pseudocamptodactyly in which wrist dorsiflexion, but not volarflexion, produces involuntary flexion contracture of distal and proximal interphalangeal joints. Such hand and jaw contractures are caused by shortened flexor muscle-tendon units. Similar lower-limb contractures also produce foot deformity. The trismus-pseudocamptodactyly syndrome is a morbid autosomal dominant trait with variable expressivity but high penetrance. In these patients, trismus complicates dental care, feeding during infancy, and intubation for anesthesia, and the pseudocamptodactyly impairs manual dexterity, with consequent occupational and social disability. Many patients require surgical correction of contractures.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; cytosol; muscle myosin complex; sarcomere

Molecular Function: actin filament binding; ATP binding; ATPase activity; calmodulin binding; microfilament motor activity; myosin light chain binding; myosin phosphatase activity; structural constituent of muscle

Biological Process: ATP metabolic process; muscle contraction; muscle filament sliding; protein amino acid dephosphorylation; skeletal muscle contraction

Disease: Arthrogryposis, Distal, Type 7; Carney Complex Variant