Rat Mucin-2 (MUC2) ELISA Kit | MUC2 elisa kit

Rat Mucin-2 (MUC2) ELISA Kit

Cat. Num. AAA287530

• Gene Names: Muc2; MLP; HH-Muc
• Reactivity: Rat
• Synonyms: Mucin-2 (MUC2); Rat Mucin-2 (MUC2) ELISA Kit; MLP; SMUC; mucin 2; intestinal/tracheal; mucin-like protein; MUC2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat MUC2. No significant cross-reactivity or interference between Rat MUC2 and analogues was observed.
• Sequence Length: 1513

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MUC2 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate MUC2 in samples. An antibody specific for MUC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lining into the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins, polymerizes into a gel of which 80% by weight is oligosaccharide side chains which are added as post-translational modifications to the mucin proteins. This gel provides an insoluble mucous barrier that serves to protect the intestinal epithelium.
The mucin 2 protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

NCBI and Uniprot Product Information

• NCBI GI #: 27805612
• NCBI GeneID: 24572
• UniProt Accession #: Q62635; P98089
• Molecular Weight: 166,038 Da
• NCBI Official Full Name: Mucin-2
• NCBI Official Synonym Full Names: mucin 2, oligomeric mucus/gel-forming
• NCBI Official Symbol: Muc2
• NCBI Official Synonym Symbols: MLP; HH-Muc
• NCBI Protein Information: mucin-2; LOW QUALITY PROTEIN: mucin-2; intestinal mucin-like protein
• UniProt Protein Name: Mucin-2
• Protein Family: Mucin
• UniProt Gene Name: Muc2
• UniProt Synonym Gene Names: MUC-2

NCBI Description

an intestinal secretory mucin which forms dimers [RGD, Feb 2006]

Uniprot Description

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer ().