Rat Microfibrillar-associated protein 3-like (MFAP3L) ELISA Kit | MFAP3L elisa kit
Rat Microfibrillar-associated protein 3-like (MFAP3L) ELISA Kit
Reactivity
Rat
Synonyms
Microfibrillar-associated protein 3-like (MFAP3L); Rat Microfibrillar-associated protein 3-like (MFAP3L) ELISA Kit; NYD-sp9; MFAP3L elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat MFAP3L. No significant cross-reactivity or interference between Rat MFAP3L and analogues was observed.
Sequence Length
409
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MFAP3L elisa kit
Description: This assay employs a two-site sandwich ELISA to quantitate MFAP3L in samples. An antibody specific for MFAP3L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFAP3L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFAP3L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFAP3L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: NYD-SP9 was believed to be involved in spermatogenesis. Southern blot hybridization results showed that NYD-SP9 expressed highly in testis but low in ovary. Protein motif analysis of this cDNA sequence revealed a cluster of phosphorylation sites, indicating its potential involvement in signal pathways during spermatogenesis.
Furthermore, one transmembrane helix was predicted in N-terminal region, indicating that putative NYD-SP6 may be served as a transmembrane protein. The proximity of these potential phosphorylation sites to each other indicates that there may be interaction among these sites to regulate spermatogenesis. These findings suggested that protein kinase NYD-SP9 might play a role in male germ cell differentiation.
Overview: NYD-SP9 was believed to be involved in spermatogenesis. Southern blot hybridization results showed that NYD-SP9 expressed highly in testis but low in ovary. Protein motif analysis of this cDNA sequence revealed a cluster of phosphorylation sites, indicating its potential involvement in signal pathways during spermatogenesis.
Furthermore, one transmembrane helix was predicted in N-terminal region, indicating that putative NYD-SP6 may be served as a transmembrane protein. The proximity of these potential phosphorylation sites to each other indicates that there may be interaction among these sites to regulate spermatogenesis. These findings suggested that protein kinase NYD-SP9 might play a role in male germ cell differentiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,377 Da
NCBI Official Full Name
microfibrillar-associated protein 3-like
NCBI Official Synonym Full Names
microfibril associated protein 3 like
NCBI Official Symbol
Mfap3l
NCBI Protein Information
microfibrillar-associated protein 3-like
UniProt Protein Name
Microfibrillar-associated protein 3-like
Protein Family
UniProt Gene Name
Mfap3l
Uniprot Description
May participate in the nuclear signaling of EGFR and MAPK1/ERK2.
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Product Notes
The Rat MFAP3L mfap3l (Catalog #AAA287548) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287548 ELISA Kit recognizes Rat MFAP3L. It is sometimes possible for the material contained within the vial of "Microfibrillar-associated protein 3-like (MFAP3L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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