Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat MAP kinase-activated protein kinase 3 (MAPKAPK3) Standard Curve.)
Rat MAP Kinase-Activated Protein Kinase 3 (MAPKAPK3) ELISA Kit | MAPKAPK3 elisa kit
Rat MAP Kinase-Activated Protein Kinase 3 (MAPKAPK3) ELISA Kit
Gene Names
Mapkapk3; MK-3; MAPKAP-K3
Reactivity
Rat
Synonyms
MAP Kinase-Activated Protein Kinase 3 (MAPKAPK3); Rat MAP Kinase-Activated Protein Kinase 3 (MAPKAPK3) ELISA Kit; MAPKAPK3; 3PK; MAPKAP3; MAPKAP kinase 3; OTTHUMP00000210806; MAPKAPK3 elisa kit
Reactivity
Rat
Specificity
Rat MAP kinase-activated protein kinase 3 (MAPKAPK3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAPKAPK3. No significant cross-reactivity or interference between Rat MAPKAPK3 and analogues was observed.
Sequence Length
384
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat MAP kinase-activated protein kinase 3 (MAPKAPK3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat MAP kinase-activated protein kinase 3 (MAPKAPK3) Standard Curve.)
Related Product Information for MAPKAPK3 elisa kit
Description: This Rat MAP kinase-activated protein kinase 3 (MAPKAPK3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAPK3 in samples. An antibody specific for MAPKAPK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPKAPK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAPK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAPK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAPKAPK3 functions as a mitogen-activated protein kinase (MAP kinase)-activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.
Background: MAPKAPK3 functions as a mitogen-activated protein kinase (MAP kinase)-activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
MAP kinase-activated protein kinase 3
NCBI Official Synonym Full Names
mitogen-activated protein kinase-activated protein kinase 3
NCBI Official Symbol
Mapkapk3
NCBI Official Synonym Symbols
MK-3; MAPKAP-K3
NCBI Protein Information
MAP kinase-activated protein kinase 3
UniProt Protein Name
MAP kinase-activated protein kinase 3
Protein Family
UniProt Gene Name
Mapkapk3
UniProt Synonym Gene Names
MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3
UniProt Entry Name
MAPK3_RAT
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Product Notes
The Rat MAPKAPK3 mapkapk3 (Catalog #AAA9712327) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712327 ELISA Kit recognizes Rat MAPKAPK3. It is sometimes possible for the material contained within the vial of "MAP Kinase-Activated Protein Kinase 3 (MAPKAPK3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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