Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Lamin A/C (LMNA) Standard Curve.)
Rat Lamin A/C (LMNA) ELISA Kit | LMNA elisa kit
Rat Lamin A/C (LMNA) ELISA Kit
Reactivity
Rat
Synonyms
Lamin A/C (LMNA); Rat Lamin A/C (LMNA) ELISA Kit; LMNA; RP11-54H19.1; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; PRO1; 70 kDa lamin; OTTHUMP00000015843; LMNA elisa kit
Reactivity
Rat
Specificity
Rat Lamin A/C (LMNA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LMNA. No significant cross-reactivity or interference between Rat LMNA and analogues was observed.
Sequence Length
665
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Lamin A/C (LMNA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Lamin A/C (LMNA) Standard Curve.)
Related Product Information for LMNA elisa kit
Description: This Rat Lamin A/C (LMNA) ELISA Kit employs a two-site sandwich ELISA to quantitate LMNA in samples. An antibody specific for LMNA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LMNA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LMNA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMNA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. Lamin A is targeted to the nuclear membrane by an isoprenyl group but it is cleaved shortly after arriving at the membrane. It stays associated with the membrane through protein-protein interactions of itself and other membrane associated proteins, such as LAP1. Depolymerization of the nuclear lamins leads to disintegration of the nuclear envelope. Transfection experiments demonstrate that phosphorylation of human lamin A is required for lamin depolymerization, and thus for disassembly of the nuclear envelope, which normally occurs early in mitosis.
Background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. Lamin A is targeted to the nuclear membrane by an isoprenyl group but it is cleaved shortly after arriving at the membrane. It stays associated with the membrane through protein-protein interactions of itself and other membrane associated proteins, such as LAP1. Depolymerization of the nuclear lamins leads to disintegration of the nuclear envelope. Transfection experiments demonstrate that phosphorylation of human lamin A is required for lamin depolymerization, and thus for disassembly of the nuclear envelope, which normally occurs early in mitosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Prelamin-A/C
NCBI Official Synonym Full Names
lamin A/C
NCBI Official Symbol
Lmna
NCBI Protein Information
prelamin-A/C
UniProt Protein Name
Prelamin-A/C
Protein Family
UniProt Gene Name
Lmna
UniProt Synonym Gene Names
Lmn1
UniProt Entry Name
LMNA_RAT
NCBI Description
a component of the nuclear membrane on the nucleoplasmic side or lamina [RGD, Feb 2006]
Research Articles on LMNA
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Product Notes
The Rat LMNA lmna (Catalog #AAA9712360) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712360 ELISA Kit recognizes Rat LMNA. It is sometimes possible for the material contained within the vial of "Lamin A/C (LMNA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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