Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Klotho (KL) Standard Curve.)
Rat Klotho (KL) ELISA Kit | KL elisa kit
Rat Klotho (KL) ELISA Kit
Reactivity
Rat
Synonyms
Klotho (KL); Rat Klotho (KL) ELISA Kit; KL elisa kit
Reactivity
Rat
Specificity
Rat Klotho (KL) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KL. No significant cross-reactivity or interference between Rat KL and analogues was observed.
Sequence Length
1014
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Klotho (KL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Klotho (KL) Standard Curve.)
Related Product Information for KL elisa kit
Description: This Rat Klotho (KL) ELISA Kit employs a two-site sandwich ELISA to quantitate KL in samples. An antibody specific for KL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Klotho is a type-I membrane protein that is related to beta-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. Klotho is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. Its discovery was documented in 1997 by Kuro-o et al. The name of the gene comes from Klotho or Clotho, one of the Moirae, or Fates, in Greek mythology.
Background: Klotho is a type-I membrane protein that is related to beta-glucuronidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. Klotho is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. Its discovery was documented in 1997 by Kuro-o et al. The name of the gene comes from Klotho or Clotho, one of the Moirae, or Fates, in Greek mythology.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
klotho
NCBI Official Synonym Full Names
Klotho
NCBI Official Symbol
Kl
NCBI Protein Information
klotho
UniProt Protein Name
Klotho
Protein Family
UniProt Gene Name
Kl
UniProt Entry Name
KLOT_RAT
NCBI Description
may be involved in suprresion of aging phenotypes [RGD, Feb 2006]
Research Articles on KL
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Product Notes
The Rat KL kl (Catalog #AAA9712389) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712389 ELISA Kit recognizes Rat KL. It is sometimes possible for the material contained within the vial of "Klotho (KL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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