Rat Interleukin 8 Receptor Alpha (IL8Ra) ELISA Kit | IL8Ra elisa kit

Rat Interleukin 8 Receptor Alpha (IL8Ra) ELISA Kit

Cat. Num. AAA2700768

• Gene Names: CXCR1; C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1
• Reactivity: Rat
• Synonyms: Interleukin 8 Receptor Alpha (IL8Ra); Rat Interleukin 8 Receptor Alpha (IL8Ra) ELISA Kit; CD181; CDw128a; CXCR1; CKR1; CMKAR1; IL8R1; IL8RBA; IL8-RA; Chemokine C-X-C motif Receptor 1; High Affinity Interleukin-8 Receptor A; IL8Ra elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 8 Receptor Alpha (IL8Ra).; No significant cross-reactivity or interference between Interleukin 8 Receptor Alpha (IL8Ra) and analogues was observed.
• Sequence Length: 350

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 Receptor Alpha (IL8Ra) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 Receptor Alpha (IL8Ra) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL8Ra elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Alpha (IL8Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 Receptor Alpha (IL8Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Alpha (IL8Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Alpha (IL8Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 559050
• NCBI GeneID: 3577
• UniProt Accession #: P25024; Q2YEF8; Q2YEG4; Q2YEG5; Q2YEG7; Q2YEG8; Q53R18; Q6IN95; Q8N6T6; Q9P2T8; Q9P2T9; B2R6Q3
• Molecular Weight: 39,791 Da
• NCBI Official Full Name: interleukin 8 receptor alpha
• NCBI Official Synonym Full Names: C-X-C motif chemokine receptor 1
• NCBI Official Symbol: CXCR1
• NCBI Official Synonym Symbols: C-C; CD128; CD181; CKR-1; IL8R1; IL8RA; CMKAR1; IL8RBA; CDw128a; C-C-CKR-1
• NCBI Protein Information: C-X-C chemokine receptor type 1
• UniProt Protein Name: C-X-C chemokine receptor type 1
• UniProt Gene Name: CXCR1
• UniProt Synonym Gene Names: CMKAR1; IL8RA; CXC-R1; CXCR-1; IL-8R A

NCBI Description

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.