Typical Testing Data/Standard Curve (for reference only)
Rat Interferon Alpha 13 (IFNa13) ELISA Kit | IFNa13 elisa kit
Rat Interferon Alpha 13 (IFNa13) ELISA Kit
Reactivity
Rat
Synonyms
Interferon Alpha 13 (IFNa13); Rat Interferon Alpha 13 (IFNa13) ELISA Kit; LeIF D; Interferon alpha-D; IFNa13 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Alpha 13 (IFNa13).
No significant cross-reactivity or interference between Interferon Alpha 13 (IFNa13) and analogues was observed.
No significant cross-reactivity or interference between Interferon Alpha 13 (IFNa13) and analogues was observed.
Sequence Length
189
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
7.81-500pg/mL
Sensitivity
< 2.8pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Interferon Alpha 13 (IFNa13) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Interferon Alpha 13 (IFNa13) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IFNa13 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 13 (IFNa13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha 13 (IFNa13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha 13 (IFNa13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Alpha 13 (IFNa13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for IFNa13 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,725 Da
NCBI Official Full Name
interferon-alpha 13
NCBI Official Synonym Full Names
interferon, alpha 13
NCBI Official Symbol
IFNA13
NCBI Protein Information
interferon alpha-1/13
UniProt Protein Name
Interferon alpha-1/13
Protein Family
UniProt Gene Name
IFNA1
UniProt Synonym Gene Names
IFN-alpha-1/13; LeIF D
UniProt Entry Name
IFNA1_HUMAN
Uniprot Description
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular space; extracellular region
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response
Research Articles on IFNa13
1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
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Product Notes
The Rat IFNa13 ifna1 (Catalog #AAA2021515) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021515 ELISA Kit recognizes Rat IFNa13. It is sometimes possible for the material contained within the vial of "Interferon Alpha 13 (IFNa13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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