Rat IGFBP5 ELISA Kit | IGFBP5 elisa kit

Rat IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit

Cat. Num. AAA2501845

• Gene Names: IGFBP5; IBP5
• Reactivity: Rat
• Synonyms: IGFBP5; Rat IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit; IGFBP5 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat IGFBP5. No significant cross-reactivity or interference between Rat IGFBP5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat IGFBP5 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 272

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP5 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP5 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IGFBP5. You can calculate the concentration of Rat IGFBP5 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10834982
• NCBI GeneID: 3488
• NCBI Accession #: NP_000590.1
• NCBI GenBank Nucleotide #: NM_000599.3
• UniProt Accession #: P24593; Q5U0A3
• Molecular Weight: 30,570 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: IGFBP5
• NCBI Official Synonym Symbols: IBP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP5
• UniProt Synonym Gene Names: IBP5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Entry Name: IBP5_HUMAN

Uniprot Description

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region

Molecular Function: protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; signal transduction; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; negative regulation of translation; striated muscle cell differentiation; negative regulation of osteoblast differentiation; regulation of cell growth; skeletal muscle growth; negative regulation of cell migration