Rat High Density Lipoprotein Binding Protein (HDLBP) ELISA Kit | HDLBP elisa kit

Rat High Density Lipoprotein Binding Protein (HDLBP) ELISA Kit

Cat. Num. AAA2704510

• Gene Names: HDLBP; HBP; VGL; PRO2900
• Reactivity: Rat
• Synonyms: High Density Lipoprotein Binding Protein (HDLBP); Rat High Density Lipoprotein Binding Protein (HDLBP) ELISA Kit; HBP; VGL; Vigilin; HDLBP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Density Lipoprotein Binding Protein (HDLBP).; No significant cross-reactivity or interference between High Density Lipoprotein Binding Protein (HDLBP) and analogues was observed.
• Sequence Length: 1268

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.128ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDLBP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein Binding Protein (HDLBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Density Lipoprotein Binding Protein (HDLBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 183892
• NCBI GeneID: 3069
• Molecular Weight: 137,987 Da
• NCBI Official Full Name: high density lipoprotein binding protein
• NCBI Official Synonym Full Names: high density lipoprotein binding protein
• NCBI Official Symbol: HDLBP
• NCBI Official Synonym Symbols: HBP; VGL; PRO2900
• NCBI Protein Information: vigilin
• UniProt Protein Name: Vigilin
• Protein Family: Vigilin
• UniProt Gene Name: HDLBP
• UniProt Synonym Gene Names: HBP; VGL; HDL-binding protein

NCBI Description

The protein encoded by this gene binds high density lipoprotein (HDL) and may function to regulate excess cholesterol levels in cells. The encoded protein also binds RNA and can induce heterochromatin formation. [provided by RefSeq, Mar 2016]

Uniprot Description

vigilin: a nuclear and cytoplasmic protein that may play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.

Protein type: Lipid-binding; RNA-binding

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytosol; high-density lipoprotein particle; nucleus; plasma membrane

Molecular Function: cadherin binding; lipid binding; protein binding; RNA binding

Biological Process: cholesterol metabolic process; high-density lipoprotein particle clearance; lipid transport