Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamate receptor 2 (GRIA2) Standard Curve.)
Rat Glutamate Receptor 2 (GRIA2) ELISA Kit | GRIA2 elisa kit
Rat Glutamate Receptor 2 (GRIA2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamate receptor 2 (GRIA2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamate receptor 2 (GRIA2) Standard Curve.)
Background: GRIA2 belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene, which includes the generation of flip and flop isoforms that vary in their signal transduction properties.
NCBI and Uniprot Product Information
NCBI Description
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GluR2: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA(quisqualate) > glutamate > kainate. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated; Channel, calcium
Cellular Component: dendrite cytoplasm; cell surface; integral to plasma membrane; dendrite; integral to membrane; terminal button; postsynaptic membrane; synaptic vesicle; cell soma; membrane; synapse; dendritic shaft; endoplasmic reticulum membrane; neuron projection; protein complex; synaptic vesicle membrane; endoplasmic reticulum; postsynaptic density; dendritic spine; perikaryon; presynaptic membrane; asymmetric synapse; growth cone; ionotropic glutamate receptor complex; cell junction
Molecular Function: identical protein binding; protein binding; kainate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; calcium channel regulator activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; receptor activity; protein kinase binding; PDZ domain binding
Biological Process: synaptic transmission; establishment of protein localization; regulation of synaptic transmission, glutamatergic; ionotropic glutamate receptor signaling pathway; response to lithium ion; receptor internalization; regulation of receptor recycling; positive regulation of synaptic transmission; protein tetramerization
Research Articles on GRIA2
Similar Products
Product Notes
The Rat GRIA2 gria2 (Catalog #AAA9712578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712578 ELISA Kit recognizes Rat GRIA2. It is sometimes possible for the material contained within the vial of "Glutamate Receptor 2 (GRIA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
