Rat Gap junction gamma-1 protein (GJC1) ELISA Kit | GJC1 elisa kit

Rat Gap junction gamma-1 protein (GJC1) ELISA Kit

Cat. Num. AAA7229741

• Gene Names: GJC1; CX45; GJA7
• Reactivity: Rat
• Synonyms: Gap junction gamma-1 protein (GJC1); Rat Gap junction gamma-1 protein (GJC1) ELISA Kit; GJC1 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This GJC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GJC1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GJC1 elisa kit

Principle of the assay: GJC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GJC1 antibody and an GJC1-HRP conjugate. The assay sample and buffer are incubated together with GJC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GJC1 concentration since GJC1 from samples and GJC1-HRP conjugate compete for the anti-GJC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GJC1 from the sample, fewer sites are left to bind GJC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GJC1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GJC1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 122939172
• NCBI GeneID: 10052
• NCBI Accession #: NP_001073852.1
• NCBI GenBank Nucleotide #: NM_001080383.1
• Molecular Weight: 45,470 Da
• NCBI Official Full Name: gap junction gamma-1 protein
• NCBI Official Synonym Full Names: gap junction protein, gamma 1, 45kDa
• NCBI Official Symbol: GJC1
• NCBI Official Synonym Symbols: CX45; GJA7
• NCBI Protein Information: gap junction gamma-1 protein; connexin 45; connexin-45; gap junction alpha-7 protein
• UniProt Protein Name: Gap junction gamma-1 protein
• Protein Family: Gap junction gamma-1 protein
• UniProt Gene Name: GJC1
• UniProt Synonym Gene Names: GJA7; Cx45
• UniProt Entry Name: CXG1_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

GJC1: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Gamma-type subfamily.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: connexon complex; endoplasmic reticulum membrane; integral to membrane; gap junction; plasma membrane

Molecular Function: ion channel activity; gap junction channel activity

Biological Process: cardiac muscle development; intercellular junction assembly; synaptic transmission; visual perception; gap junction assembly; muscle contraction; transport; vasculogenesis; cell development