IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using c-Raf (Phospho-Ser296) antibody. Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using c-Raf (Phospho-Ser296) antibody.)
c-Raf Antibody | anti-c-Raf antibody
c-Raf (Phospho-Ser296) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
c-Raf; N/A; c-Raf (Phospho-Ser296) Antibody; C-RAF; C-Raf; CRAF; EC 2.7.11.1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; RAF-1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; Raf-1; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1; kinase Raf1; anti-c-Raf antibody
Reactivity
Human, Mouse, Rat
Specificity
c-Raf (Phospho-Ser296) antibody detects endogenous levels of c-Raf only when phosphorylated at serine 296.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
648
Applicable Applications for anti-c-Raf antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human c-Raf around the phosphorylation site of serine 296 (S-S-SP-P-N).
All Sites
Human: Ser296
Mouse: Ser296
Rat: Ser296
Mouse: Ser296
Rat: Ser296
Preparation and Storage
Store at-20 degree C for 1 year.
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using c-Raf (Phospho-Ser296) antibody. Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using c-Raf (Phospho-Ser296) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using c-Raf (Phospho-Ser296) antibody. Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using c-Raf (Phospho-Ser296) antibody.)
Application Data
(P-peptide-+)
Application Data
(P-peptide-+)
References
Marie A. Bogoyevitch, J. Biol. Chem., Nov 1995; 270: 26303. G Heidecker, Mol. Cell. Biol., Jun 1990; 10: 2503-2512. Shari L. Sutor, J. Biol. Chem., Mar 1999; 274: 7002. Albrecht Piiper, Mol. Pharmacol., Sep 2000; 58: 608.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,395 Da
NCBI Official Full Name
RAF proto-oncogene serine/threonine-protein kinase isoform b
NCBI Official Synonym Full Names
Raf-1 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
RAF1
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf; CMD1NN
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Gene Name
RAF1
UniProt Synonym Gene Names
RAF; cRaf
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Product Notes
The c-Raf raf1 (Catalog #AAA267476) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The c-Raf (Phospho-Ser296) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-Raf can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the c-Raf raf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Raf, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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