Rat Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit | FABP6 elisa kit

Rat Fatty Acid Binding Protein 6, Ileal (FABP6) ELISA Kit

Cat. Num. AAA2024759

• Gene Names: FABP6; ILBP; I-15P; I-BAP; ILBP3; ILLBP; I-BABP; I-BALB
• Reactivity: Rat
• Synonyms: Fatty Acid Binding Protein 6; Ileal (FABP6); Rat Fatty Acid Binding Protein 6; Ileal (FABP6) ELISA Kit; GT; iBABP; I-BABP; I-BALB; I-BAP; ILBP; ILBP3; ILLBP; I-15P; Gastrotropin; Illeal Lipid-Binding Protein; Ileal Bile Acid Binding Protein; Intestinal 15 kDa protein; FABP6 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 6, Ileal (FABP6).; No significant cross-reactivity or interference between Fatty Acid Binding Protein 6, Ileal (FABP6) and analogues was observed.
• Sequence Length: 248

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 78.12-5000pg/mL
• Sensitivity: < 27pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 6, Ileal (FABP6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 6, Ileal (FABP6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 6, Ileal (FABP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 6, Ileal (FABP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 6, Ileal (FABP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Binding Protein 6, Ileal (FABP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FABP6 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 119581970
• NCBI GeneID: 2172
• Molecular Weight: 19,874 Da
• NCBI Official Full Name: fatty acid binding protein 6, ileal (gastrotropin), isoform CRA_d, partial
• NCBI Official Synonym Full Names: fatty acid binding protein 6, ileal
• NCBI Official Symbol: FABP6
• NCBI Official Synonym Symbols: ILBP; I-15P; I-BAP; ILBP3; ILLBP; I-BABP; I-BALB
• NCBI Protein Information: gastrotropin
• UniProt Protein Name: Gastrotropin
• Protein Family: Gastrotropin
• UniProt Gene Name: FABP6
• UniProt Synonym Gene Names: ILBP; ILLBP; GT; ILBP; I-15P; I-BABP
• UniProt Entry Name: FABP6_HUMAN

NCBI Description

This gene encodes the ileal fatty acid binding protein. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP6 and FABP1 (the liver fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Transcript variants generated by alternate transcription promoters and/or alternate splicing have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP6: Ileal protein which stimulates gastric acid and pepsinogen secretion. Seems to be able to bind to bile salts and bilirubins. Isoform 2 is essential for the survival of colon cancer cells to bile acid-induced apoptosis. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 5q33.3-q34

Cellular Component: cytoplasm; cytosol

Molecular Function: transporter activity; lipid binding

Biological Process: bile acid and bile salt transport; negative regulation of cell proliferation; bile acid metabolic process; lipid metabolic process; transmembrane transport