Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) Standard Curve.)
Rat Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit DAD1 (DAD1) ELISA Kit | DAD1 elisa kit
Rat Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit DAD1 (DAD1) ELISA Kit
Reactivity
Rat
Synonyms
Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit DAD1 (DAD1); Rat Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit DAD1 (DAD1) ELISA Kit; DAD1; OST2; DAD1 elisa kit
Reactivity
Rat
Specificity
Rat Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DAD1. No significant cross-reactivity or interference between Rat DAD1 and analogues was observed.
Sequence Length
113
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) Standard Curve.)
Related Product Information for DAD1 elisa kit
Description: This Rat Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate DAD1 in samples. An antibody specific for DAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.
Background: DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
NCBI Official Synonym Full Names
defender against cell death 1
NCBI Official Symbol
Dad1
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Protein Family
UniProt Gene Name
Dad1
UniProt Synonym Gene Names
Oligosaccharyl transferase subunit DAD1; DAD-1
UniProt Entry Name
DAD1_RAT
NCBI Description
human homolog inhibits apoptosis [RGD, Feb 2006]
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Product Notes
The Rat DAD1 dad1 (Catalog #AAA9712472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712472 ELISA Kit recognizes Rat DAD1. It is sometimes possible for the material contained within the vial of "Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit DAD1 (DAD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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