Rat DnaJ homolog subfamily B member 11 (DNAJB11) ELISA Kit | DNAJB11 elisa kit

Rat DnaJ homolog subfamily B member 11 (DNAJB11) ELISA Kit

Cat. Num. AAA2800155

• Gene Names: Dnajb11; LRRGT00084
• Reactivity: Rat
• Synonyms: DnaJ homolog subfamily B member 11 (DNAJB11); Rat DnaJ homolog subfamily B member 11 (DNAJB11) ELISA Kit; ABBP-2; ABBP2; DJ9; EDJ; ERdj3; ERj3; HEDJ; PRO1080; UNQ537; hDj9; ER-associated DNAJ protein 3; ER-associated Hsp40 co-chaperone; OTTHUMP00000210171; PWP1-interacting protein 4; DNAJB11 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat DNAJB11. No significant cross-reactivity or interference between Rat DNAJB11 and analogues was observed.
• Sequence Length: 358

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DNAJB11 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate DNAJB11 in samples. An antibody specific for DNAJB11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAJB11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJB11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJB11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: DNAJB11 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a C-terminal cysteine-rich region.The deduced 358-amino acid protein has a calculated molecular mass of 40.5 kD and shares 99% sequence identity with the mouse protein. DNAJB11 contains a conserved J domain, a weak G/F region, and a region that contains 4 cysteines but lacks the zinc finger domain found in some DNAJ proteins. The secondary and tertiary structures of DNAJB11 closely resemble those of HDJ1 (DNAJB1). Northern blot analysis detected transcripts of 1.5 and 2.0 kb in all tissues examined.

NCBI and Uniprot Product Information

• NCBI GI #: 62543491
• NCBI GeneID: 360734
• NCBI Accession #: NP_001015021.1
• NCBI GenBank Nucleotide #: NM_001015021.1
• UniProt Accession #: Q6TUG0
• Molecular Weight: 40,495 Da
• NCBI Official Full Name: dnaJ homolog subfamily B member 11
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B11
• NCBI Official Symbol: Dnajb11
• NCBI Official Synonym Symbols: LRRGT00084
• NCBI Protein Information: dnaJ homolog subfamily B member 11
• UniProt Protein Name: DnaJ homolog subfamily B member 11
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: Dnajb11
• UniProt Synonym Gene Names: ER-resident protein ERdj3; ERdj3; ERj3p

Uniprot Description

Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity ().