Rat DNA repair protein XRCC1 ELISA Kit | XRCC1 elisa kit
Rat DNA repair protein XRCC1 (XRCC1) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XRCC1 in samples. An antibody specific for XRCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXRCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRCC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
plays a role in base-excision repair; displays decreased mRNA expression in early embryos treated with the anticancer alkylating agent cyclophosphamide [RGD, Feb 2006]
Uniprot Description
XRCC1: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: nuclear chromatin; nuclear chromosome, telomeric region; nucleolus; nucleus
Molecular Function: enzyme binding; oxidized DNA binding; protein binding
Biological Process: base-excision repair; cellular response to DNA damage stimulus; DNA repair; double-strand break repair via nonhomologous end joining; hippocampus development; response to drug; response to hypoxia; response to organic substance; single strand break repair
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Product Notes
The Rat XRCC1 xrcc1 (Catalog #AAA285175) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285175 ELISA Kit recognizes Rat XRCC1. It is sometimes possible for the material contained within the vial of "DNA repair protein XRCC1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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