Rat DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit | RAD51 elisa kit
Rat DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit
Reactivity
Rat
Synonyms
DNA repair protein RAD51 homolog 1 (RAD51); Rat DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit; BRCC5; HRAD51; HsRad51; HsT16930; RAD51A; RECA; BRCA1/BRCA2-containing complex; subunit 5|DNA repair protein RAD51 homolog 1|RAD51 homolog protein|RecA; E Coli; homolog of|RecA-like protein|recombi; RAD51 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat RAD51. No significant cross-reactivity or interference between Rat RAD51 and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate RAD51 in samples. An antibody specific for RAD51 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAD51 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD51 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD51 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
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Product Notes
The Rat RAD51 (Catalog #AAA2803240) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2803240 ELISA Kit recognizes Rat RAD51. It is sometimes possible for the material contained within the vial of "DNA repair protein RAD51 homolog 1 (RAD51), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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