Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA-3-methyladenine glycosylase (MPG) Standard Curve.)
Rat DNA-3-Methyladenine Glycosylase (MPG) ELISA Kit | MPG elisa kit
Rat DNA-3-Methyladenine Glycosylase (MPG) ELISA Kit
Reactivity
Rat
Synonyms
DNA-3-Methyladenine Glycosylase (MPG); Rat DNA-3-Methyladenine Glycosylase (MPG) ELISA Kit; MPG; AAG; APNG; CRA36.1; MDG; Mid1; PIG11; PIG16; anpg; 3 end of the Mid1 gene; localized 68 kb upstream the humanzeta globin gene on 16p; CRA36.1 (3-methyl-adenine DNA glycosylase); N-methylpurine-DNA gl; MPG elisa kit
Reactivity
Rat
Specificity
Rat DNA-3-methyladenine glycosylase (MPG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MPG. No significant cross-reactivity or interference between Rat MPG and analogues was observed.
Sequence Length
317
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA-3-methyladenine glycosylase (MPG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA-3-methyladenine glycosylase (MPG) Standard Curve.)
Related Product Information for MPG elisa kit
Description: This Rat DNA-3-methyladenine glycosylase (MPG) ELISA Kit employs a two-site sandwich ELISA to quantitate MPG in samples. An antibody specific for MPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MPG (N-Methylpurine DNA Glycosylase) is a Protein Coding gene. Among its related pathways are Recognition and association of DNA glycosylase with site containing an affected purine and Telomere C-strand (Lagging Strand) Synthesis. GO annotations related to this gene include damaged DNA binding and DNA-3-methyladenine glycosylase activity. Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Background: MPG (N-Methylpurine DNA Glycosylase) is a Protein Coding gene. Among its related pathways are Recognition and association of DNA glycosylase with site containing an affected purine and Telomere C-strand (Lagging Strand) Synthesis. GO annotations related to this gene include damaged DNA binding and DNA-3-methyladenine glycosylase activity. Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
DNA-3-methyladenine glycosylase
NCBI Official Synonym Full Names
N-methylpurine-DNA glycosylase
NCBI Official Symbol
Mpg
NCBI Protein Information
DNA-3-methyladenine glycosylase
UniProt Protein Name
DNA-3-methyladenine glycosylase
Protein Family
UniProt Gene Name
Mpg
NCBI Description
enzyme that cleaves 3-methyladenine and 7-methylguanine residues from DNA [RGD, Feb 2006]
Uniprot Description
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
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Product Notes
The Rat MPG mpg (Catalog #AAA9712249) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712249 ELISA Kit recognizes Rat MPG. It is sometimes possible for the material contained within the vial of "DNA-3-Methyladenine Glycosylase (MPG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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