Rat Dipeptidyl Peptidase IV (DPP4) ELISA Kit | DPP4 elisa kit

Rat Dipeptidyl Peptidase IV (DPP4) ELISA Kit

Cat. Num. AAA2702646

• Gene Names: DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
• Reactivity: Rat
• Synonyms: Dipeptidyl Peptidase IV (DPP4); Rat Dipeptidyl Peptidase IV (DPP4) ELISA Kit; CD26; ADA; DPPIV; DPP-IV; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26; DPP4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase IV (DPP4).; No significant cross-reactivity or interference between Dipeptidyl Peptidase IV (DPP4) and analogues was observed.
• Sequence Length: 31

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.269ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase IV (DPP4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase IV (DPP4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPP4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase IV (DPP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase IV (DPP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase IV (DPP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase IV (DPP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1195575
• NCBI GeneID: 1803
• UniProt Accession #: P27487; Q53TN1
• Molecular Weight: 88,279 Da
• NCBI Official Full Name: dipeptidyl peptidase IV, partial
• NCBI Official Synonym Full Names: dipeptidyl peptidase 4
• NCBI Official Symbol: DPP4
• NCBI Official Synonym Symbols: CD26; ADABP; ADCP2; DPPIV; TP103
• NCBI Protein Information: dipeptidyl peptidase 4
• UniProt Protein Name: Dipeptidyl peptidase 4
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: DPP4
• UniProt Synonym Gene Names: ADCP2; CD26; ADCP-2; DPP IV

NCBI Description

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.