Rat CYP21A1 (Steroid 21-hydroxylase) ELISA Kit | CYP21A1 elisa kit

Rat CYP21A1 (Steroid 21-hydroxylase) ELISA Kit

Cat. Num. AAA7607283

• Gene Names: CYP21A2; CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B
• Reactivity: Rat
• Synonyms: CYP21A1 (Steroid 21-hydroxylase); Rat CYP21A1 (Steroid 21-hydroxylase) ELISA Kit; CYP21A1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYP11B1. No significant cross-reactivity or interference between CYP11B1 and analogues was observed.
• Sequence Length: 360

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP11B1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP11B1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP21A1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CYP11B1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CYP11B1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CYP11B1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CYP11B1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1559191263
• NCBI GeneID: 1589
• NCBI Accession #: NP_001355072.1
• NCBI GenBank Nucleotide #: NM_001368143.1
• Molecular Weight: 52,597 Da
• NCBI Official Full Name: steroid 21-hydroxylase isoform c
• NCBI Official Synonym Full Names: cytochrome P450 family 21 subfamily A member 2
• NCBI Official Symbol: CYP21A2
• NCBI Official Synonym Symbols: CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B
• NCBI Protein Information: steroid 21-hydroxylase
• UniProt Protein Name: Steroid 21-hydroxylase
• UniProt Gene Name: CYP21A2
• UniProt Synonym Gene Names: CYP21; CYP21B

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids (PubMed:22014889).