Rat Cyclic ADP Ribose Hydrolase (cADPRH) ELISA Kit | cADPRH elisa kit

Rat Cyclic ADP Ribose Hydrolase (cADPRH) ELISA Kit

Cat. Num. AAA2019767

• Gene Names: CD38; T10; ADPRC 1
• Reactivity: Rat
• Synonyms: Cyclic ADP Ribose Hydrolase (cADPRH); Rat Cyclic ADP Ribose Hydrolase (cADPRH) ELISA Kit; CD38; ADPRC 1; c-ADPRH; T10; p45; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; cADPRH elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclic ADP Ribose Hydrolase (cADPRH).; No significant cross-reactivity or interference between Cyclic ADP Ribose Hydrolase (cADPRH) and analogues was observed.
• Sequence Length: 60

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.24ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclic ADP Ribose Hydrolase (cADPRH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclic ADP Ribose Hydrolase (cADPRH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for cADPRH elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclic ADP Ribose Hydrolase (cADPRH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclic ADP Ribose Hydrolase (cADPRH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclic ADP Ribose Hydrolase (cADPRH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclic ADP Ribose Hydrolase (cADPRH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for cADPRH elisa kit

Signal transduction; CD & Adhesion molecule; Enzyme & Kinase; Metabolic pathway; Tumor immunity; Infection immunity; Endocrinology; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 68989438
• NCBI GeneID: 952
• UniProt Accession #: P28907; O00121; O00122; Q96HY4
• Molecular Weight: 13,788 Da
• NCBI Official Full Name: cyclic ADP-ribose hydrolase, partial
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: T10; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN

NCBI Description

CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.99.20; Membrane protein, integral; Hydrolase; Apoptosis; Cell surface; Cell cycle regulation; EC 3.2.2.6; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Lyase

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; nucleus

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: response to drug; response to retinoic acid; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; female pregnancy; signal transduction; positive regulation of cell growth; response to estradiol stimulus; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; response to hydroperoxide; response to hypoxia; positive regulation of B cell proliferation; positive regulation of vasoconstriction; response to progesterone stimulus; negative regulation of bone resorption; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis