Rat Creatine Kinase M-Type (CKM) ELISA Kit | CKM elisa kit
Rat Creatine Kinase M-Type (CKM) ELISA Kit
Gene Names
CKM; CPK-M
Reactivity
Rat
Synonyms
Creatine Kinase M-Type (CKM); Rat Creatine Kinase M-Type (CKM) ELISA Kit; CKM; CKMM; M-CK; creatine kinase M chain; creatine kinase-M; muscle creatine kinase; CKM elisa kit
Reactivity
Rat
Specificity
Rat Creatine kinase M-type (CKM) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CKM. No significant cross-reactivity or interference between Rat CKM and analogues was observed.
Sequence Length
381
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Creatine kinase M-type (CKM) Standard Curve.)
Related Product Information for CKM elisa kit
Description: This Rat Creatine kinase M-type (CKM) ELISA Kit employs a two-site sandwich ELISA to quantitate CKM in samples. An antibody specific for CKM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CKM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CKM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CKM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: CKM is a cytoplasmic enzyme involved in cellular energy homeostasis. The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine and between phospho-creatine and ADP. Its functional entity is a MM-CK homodimer in striated (sarcomeric) skeletal and cardiac muscle.In heart, in addition to the MM-CK homodimer also the heterodimer consisting of one muscle (MM-CK) and one brain-type (MB-CK) subunit is expressed. This latter is an important serum marker for myocardial infarction, for it is released from damaged myocardial cells into the blood where it can be detected by clinical chemistry. Since no other organ than cardiac muscle expresses significant levels of MB-CK, this clinical test is still fairly reliable, together with troponin-T, for identifying a myocardial infarction.
Background: CKM is a cytoplasmic enzyme involved in cellular energy homeostasis. The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine and between phospho-creatine and ADP. Its functional entity is a MM-CK homodimer in striated (sarcomeric) skeletal and cardiac muscle.In heart, in addition to the MM-CK homodimer also the heterodimer consisting of one muscle (MM-CK) and one brain-type (MB-CK) subunit is expressed. This latter is an important serum marker for myocardial infarction, for it is released from damaged myocardial cells into the blood where it can be detected by clinical chemistry. Since no other organ than cardiac muscle expresses significant levels of MB-CK, this clinical test is still fairly reliable, together with troponin-T, for identifying a myocardial infarction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
creatine kinase M-type
NCBI Official Symbol
CKM
NCBI Official Synonym Symbols
CPK-M
NCBI Protein Information
creatine kinase M-type
UniProt Protein Name
Creatine kinase M-type
Protein Family
UniProt Gene Name
CKM
Uniprot Description
CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.
Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoplasm; extracellular space
Molecular Function: ATP binding; creatine kinase activity
Biological Process: phosphocreatine biosynthetic process; phosphorylation; response to heat
