Rat Creatine Kinase M-Type (CKM) ELISA Kit | CKM elisa kit
Rat Creatine Kinase M-Type (CKM) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Background: CKM is a cytoplasmic enzyme involved in cellular energy homeostasis. The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine and between phospho-creatine and ADP. Its functional entity is a MM-CK homodimer in striated (sarcomeric) skeletal and cardiac muscle.In heart, in addition to the MM-CK homodimer also the heterodimer consisting of one muscle (MM-CK) and one brain-type (MB-CK) subunit is expressed. This latter is an important serum marker for myocardial infarction, for it is released from damaged myocardial cells into the blood where it can be detected by clinical chemistry. Since no other organ than cardiac muscle expresses significant levels of MB-CK, this clinical test is still fairly reliable, together with troponin-T, for identifying a myocardial infarction.
NCBI and Uniprot Product Information
Uniprot Description
CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.
Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoplasm; extracellular space
Molecular Function: ATP binding; creatine kinase activity
Biological Process: phosphocreatine biosynthetic process; phosphorylation; response to heat