Rat Complement fragment 3d (C3d) ELISA Kit | C3d elisa kit

Rat Complement fragment 3d (C3d) ELISA Kit

Cat. Num. AAA2600292

• Reactivity: Rat
• Synonyms: Complement fragment 3d (C3d); Rat Complement fragment 3d (C3d) ELISA Kit; C3d elisa kit

• Reactivity: Rat
• Sequence Length: 688

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C3d elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat C3d monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 115205
• NCBI GeneID: 716
• Molecular Weight: 76,684 Da
• NCBI Official Full Name: Complement C1s subcomponent
• NCBI Official Synonym Full Names: complement component 1, s subcomponent
• NCBI Official Symbol: C1S
• NCBI Protein Information: complement C1s subcomponent; C1 esterase; basic proline-rich peptide IB-1; complement component 1 subcomponent s
• UniProt Protein Name: Complement C1s subcomponent
• UniProt Gene Name: C1S
• UniProt Entry Name: C1S_HUMAN

NCBI Description

This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq, Mar 2009]

Uniprot Description

C1S: C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. Defects in C1S are the cause of complement component C1s deficiency (C1SD). A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.42; Protease

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; serine-type endopeptidase activity; calcium ion binding

Biological Process: innate immune response; proteolysis; complement activation, classical pathway; complement activation

Disease: Complement Component C1s Deficiency