Rat Cofilin 1, Non Muscle (CFL1) ELISA Kit | CFL1 elisa kit

Rat Cofilin 1, Non Muscle (CFL1) ELISA Kit

Cat. Num. AAA2703435

• Gene Names: CFL1; CFL; cofilin; HEL-S-15
• Reactivity: Rat
• Synonyms: Cofilin 1; Non Muscle (CFL1); Rat Cofilin 1; Non Muscle (CFL1) ELISA Kit; CFL; p18; N-Cofilin; 18 kDa phosphoprotein; CFL1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cofilin 1, Non Muscle (CFL1).; No significant cross-reactivity or interference between Cofilin 1, Non Muscle (CFL1) and analogues was observed.
• Sequence Length: 166

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cofilin 1, Non Muscle (CFL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cofilin 1, Non Muscle (CFL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cofilin 1, Non Muscle (CFL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cofilin 1, Non Muscle (CFL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cofilin 1, Non Muscle (CFL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cofilin 1, Non Muscle (CFL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5031635
• NCBI GeneID: 1072
• NCBI Accession #: NP_005498.1
• NCBI GenBank Nucleotide #: NM_005507.2
• UniProt Accession #: P23528; Q53Y87; Q9UCA2; B3KUQ1
• Molecular Weight: 18,502 Da
• NCBI Official Full Name: cofilin-1
• NCBI Official Synonym Full Names: cofilin 1
• NCBI Official Symbol: CFL1
• NCBI Official Synonym Symbols: CFL; cofilin; HEL-S-15
• NCBI Protein Information: cofilin-1
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: CFL1
• UniProt Synonym Gene Names: CFL; p18

NCBI Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Uniprot Description

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cortical actin cytoskeleton; cytoplasm; cytosol; extracellular exosome; extracellular matrix; extracellular space; focal adhesion; intercellular junction; lamellipodium; membrane; nuclear matrix; nucleus; vesicle

Molecular Function: actin filament binding; protein binding; receptor binding

Biological Process: actin cytoskeleton organization; actin filament depolymerization; actin filament fragmentation; cytoskeleton organization; establishment of cell polarity; mitotic cytokinesis; negative regulation of apoptosis; neural crest cell migration; neural fold formation; positive regulation by host of viral process; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid; response to virus; Rho protein signal transduction