Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cell division cycle 5-like protein (CDC5L) Standard Curve.)
Rat Cell Division Cycle 5-Like Protein (CDC5L) ELISA Kit | CDC5L elisa kit
Rat Cell Division Cycle 5-Like Protein (CDC5L) ELISA Kit
Reactivity
Rat
Synonyms
Cell Division Cycle 5-Like Protein (CDC5L); Rat Cell Division Cycle 5-Like Protein (CDC5L) ELISA Kit; CDC5L; RP1-319D22.1; CDC5-LIKE; CEF1; KIAA0432; PCDC5RP; dJ319D22.1; hCDC5; CDC5 (cell division cycle 5; S. pombe; homolog)-like; CDC5-like; Cdc5-related protein; Cell division cycle 5; homolog-like; CDC5L elisa kit
Reactivity
Rat
Specificity
Rat Cell division cycle 5-like protein (CDC5L) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CDC5L. No significant cross-reactivity or interference between Rat CDC5L and analogues was observed.
Sequence Length
802
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cell division cycle 5-like protein (CDC5L) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cell division cycle 5-like protein (CDC5L) Standard Curve.)
Related Product Information for CDC5L elisa kit
Description: This Rat Cell division cycle 5-like protein (CDC5L) ELISA Kit employs a two-site sandwich ELISA to quantitate CDC5L in samples. An antibody specific for CDC5L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDC5L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDC5L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDC5L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by CDC5L shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
Background: The protein encoded by CDC5L shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cell division cycle 5-like protein
NCBI Official Synonym Full Names
cell division cycle 5-like
NCBI Official Symbol
Cdc5l
NCBI Protein Information
cell division cycle 5-like protein
UniProt Protein Name
Cell division cycle 5-like protein
Protein Family
UniProt Gene Name
Cdc5l
UniProt Synonym Gene Names
Pcdc5rp
UniProt Entry Name
CDC5L_RAT
NCBI Description
prolactin-responsive gene; may be involved with pre-mRNA splicing [RGD, Feb 2006]
Similar Products
Product Notes
The Rat CDC5L cdc5l (Catalog #AAA9712498) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712498 ELISA Kit recognizes Rat CDC5L. It is sometimes possible for the material contained within the vial of "Cell Division Cycle 5-Like Protein (CDC5L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
