Rat Brain Natriuretic Peptide (BNP) ELISA Kit | BNP elisa kit

Rat Brain Natriuretic Peptide (BNP) Wide-range ELISA Kit

Cat. Num. AAA2709276

• Gene Names: Nppb; BNP; Bnf
• Reactivity: Rat
• Applications: ELISA
• Synonyms: Brain Natriuretic Peptide (BNP); Rat Brain Natriuretic Peptide (BNP) Wide-range ELISA Kit; Brain Natriuretic Peptide; GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32; BNP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Brain Natriuretic Peptide (BNP).; No significant cross-reactivity or interference between Wide-range Brain Natriuretic Peptide (BNP) and analogues was observed.
• Sequence Length: 121

• Applicable Applications for BNP elisa kit: ELISA (EIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 24.69-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 9.37pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Brain Natriuretic Peptide (BNP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Brain Natriuretic Peptide (BNP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Brain Natriuretic Peptide (BNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Brain Natriuretic Peptide (BNP) and unlabeled Wide-range Brain Natriuretic Peptide (BNP) (Standards or samples) with the pre-coated antibody specific to Wide-range Brain Natriuretic Peptide (BNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Brain Natriuretic Peptide (BNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Brain Natriuretic Peptide (BNP) in the sample.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 13928738
• NCBI GeneID: 25105
• NCBI Accession #: NP_113733.1
• NCBI GenBank Nucleotide #: NP_113733.1
• UniProt Accession #: P13205
• NCBI Official Full Name: natriuretic peptides B
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: Nppb
• NCBI Official Synonym Symbols: BNP; Bnf
• NCBI Protein Information: natriuretic peptides B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: Nppb
• UniProt Entry Name: ANFB_RAT

NCBI Description

hormone produced primarily by the atrium and ventricle of the heart [RGD, Feb 2006]

Uniprot Description

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 ().