Rat Bone Morphogenetic Protein 8A (BMP8A) ELISA Kit | BMP8A elisa kit

Rat Bone Morphogenetic Protein 8A (BMP8A) ELISA Kit

Cat. Num. AAA2704177

• Reactivity: Rat
• Synonyms: Bone Morphogenetic Protein 8A (BMP8A); Rat Bone Morphogenetic Protein 8A (BMP8A) ELISA Kit; BMP8-A; BMP8A elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 8A (BMP8A).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 8A (BMP8A) and analogues was observed.
• Sequence Length: 402

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 8A (BMP8A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 8A (BMP8A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP8A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 8A (BMP8A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 8A (BMP8A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 8A (BMP8A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 145611428
• NCBI GeneID: 353500
• NCBI Accession #: NP_861525.2
• NCBI GenBank Nucleotide #: NM_181809.3
• UniProt Accession #: Q7Z5Y6; Q5T3A5
• Molecular Weight: 44,798 Da
• NCBI Official Full Name: bone morphogenetic protein 8A preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 8a
• NCBI Official Symbol: BMP8A
• NCBI Protein Information: bone morphogenetic protein 8A
• UniProt Protein Name: Bone morphogenetic protein 8A
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP8A
• UniProt Synonym Gene Names: BMP-8A

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (). Signaling protein involved in regulation of thermogenesis and energy balance. Proposed to increase the peripheral response of brown adipose tissue (BAT) to adrenergic stimulation while acting centrally in the hypothalamus to increase sympathetic output to BAT.