Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Bone marrow proteoglycan (PRG2) Standard Curve.)
Rat Bone Marrow Proteoglycan (PRG2) ELISA Kit | PRG2 elisa kit
Rat Bone Marrow Proteoglycan (PRG2) ELISA Kit
Reactivity
Rat
Synonyms
Bone Marrow Proteoglycan (PRG2); Rat Bone Marrow Proteoglycan (PRG2) ELISA Kit; PRG2; BMPG; MBP; MBP1; MGC14537; bone-marrow proteoglycan; eosinophil granule major basic protein; eosinophil major basic protein; natural killer cell activator; proteoglycan 2; proteoglycan 2 preproprotein; PRG2 elisa kit
Reactivity
Rat
Specificity
Rat Bone marrow proteoglycan (PRG2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRG2. No significant cross-reactivity or interference between Rat PRG2 and analogues was observed.
Sequence Length
227
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Bone marrow proteoglycan (PRG2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Bone marrow proteoglycan (PRG2) Standard Curve.)
Related Product Information for PRG2 elisa kit
Description: This Rat Bone marrow proteoglycan (PRG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG2 in samples. An antibody specific for PRG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Major basic protein The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases.PRG2 is a 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro.
Background: Major basic protein The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases.PRG2 is a 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
bone marrow proteoglycan
NCBI Official Synonym Full Names
proteoglycan 2
NCBI Official Symbol
Prg2
NCBI Protein Information
bone marrow proteoglycan
UniProt Protein Name
Bone marrow proteoglycan
Protein Family
UniProt Gene Name
Prg2
UniProt Synonym Gene Names
Mbp; BMPG; EMBP; MBP
UniProt Entry Name
PRG2_RAT
NCBI Description
human homolog is the primary component of the eosinophil granule crystalline core; may play a role in allergic response and asthma [RGD, Feb 2006]
Uniprot Description
PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Protein type: Secreted, signal peptide; Secreted; Vesicle
Molecular Function: heparin binding; carbohydrate binding
Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; defense response to bacterium; immune response; defense response to nematode
Research Articles on PRG2
Similar Products
Product Notes
The Rat PRG2 prg2 (Catalog #AAA9712081) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712081 ELISA Kit recognizes Rat PRG2. It is sometimes possible for the material contained within the vial of "Bone Marrow Proteoglycan (PRG2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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