Rat B-Cell Activating Factor (BAFF) ELISA Kit | BAFF elisa kit

Rat B-Cell Activating Factor (BAFF) ELISA Kit

Cat. Num. AAA2024157

• Gene Names: TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• Reactivity: Rat
• Synonyms: B-Cell Activating Factor (BAFF); Rat B-Cell Activating Factor (BAFF) ELISA Kit; CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of BAFF. No significant cross-reactivity or interference between BAFF and analogues was observed.
• Sequence Length: 164

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 28pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BAFF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BAFF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BAFF elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BAFF in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to BAFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BAFF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BAFF elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 4585275
• NCBI GeneID: 10673
• UniProt Accession #: Q9Y275; Q6FHD6; Q7Z5J2; E0ADT7
• Molecular Weight: 17,578 Da
• NCBI Official Full Name: B-cell activating factor
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13b
• NCBI Official Symbol: TNFSF13B
• NCBI Official Synonym Symbols: DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13B
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13B
• UniProt Gene Name: TNFSF13B
• UniProt Synonym Gene Names: BAFF; BLYS; TALL1; TNFSF20; ZTNF4; BLyS; TALL-1
• UniProt Entry Name: TN13B_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

BAFF: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Apoptosis; Cytokine; Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q32-q34

Cellular Component: extracellular space; perinuclear region of cytoplasm; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; B cell costimulation; B cell homeostasis; immunoglobulin secretion; T cell costimulation; positive regulation of cell proliferation; positive regulation of germinal center formation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; immune response; signal transduction