Rat Anti-nuclear antibody (ANA) ELISA Kit | ANA elisa kit

Rat Anti-nuclear antibody (ANA) ELISA Kit

Cat. Num. AAA269217

• Gene Names: Arid3a; Dri1; Dril1; Bright
• Reactivity: Rat
• Synonyms: Anti-nuclear antibody (ANA); Rat Anti-nuclear antibody (ANA) ELISA Kit; ANA elisa kit

• Reactivity: Rat
• Sequence Length: 601

• Samples: Rat serum, plasma, or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for ANA elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antigen Sandwich ELISA technique. The pre-coated antigen is a related-Rat ANA antigen, while the detection antigen is another antigen. Samples are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 12230032
• NCBI GeneID: 13496
• Molecular Weight: 64,173 Da
• NCBI Official Full Name: AT-rich interactive domain-containing protein 3A
• NCBI Official Synonym Full Names: AT rich interactive domain 3A (BRIGHT-like)
• NCBI Official Symbol: Arid3a
• NCBI Official Synonym Symbols: Dri1; Dril1; Bright
• NCBI Protein Information: AT-rich interactive domain-containing protein 3A; dead ringer homolog 1; dead ringer-like protein 1; ARID domain-containing protein 3A; B-cell regulator of IgH transcription; AT rich interactive domain 3A (Bright like)
• UniProt Protein Name: AT-rich interactive domain-containing protein 3A
• Protein Family: Protein anachronism
• UniProt Gene Name: Arid3a
• UniProt Synonym Gene Names: Dri1; Dril1; ARID domain-containing protein 3A; Bright
• UniProt Entry Name: ARI3A_MOUSE

Uniprot Description

Bright: a member of the ARID (AT-rich interaction domain) family of transcription factors. It was found by its homology to the Drosophila dead ringer protein, which is important for normal embryogenesis. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. Contributes to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination.

Protein type: Transcription factor

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; nucleus; lipid raft

Molecular Function: protein binding; protein homodimerization activity; DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter