Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Androgen Standard Curve.)
Rat Androgen ELISA Kit
Rat Androgen ELISA Kit
Reactivity
Rat
Synonyms
Androgen; Rat Androgen ELISA Kit; Androgen elisa kit
Reactivity
Rat
Specificity
Rat Androgen ELISA Kit has high sensitivity and excellent specificity for detection of Rat Androgen. No significant cross-reactivity or interference between Rat Androgen and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Androgen Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Androgen Standard Curve.)
Related Product Information for Androgen elisa kit
Description: This Rat Androgen ELISA Kit employs a two-site sandwich ELISA to quantitate Androgen in samples. An antibody specific for Androgen has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Androgen present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Androgen is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Androgen bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: An androgen (from Greek andro meaning male human being) is any natural or synthetic compound, usually a steroid hormone, that stimulates or controls the development and maintenance of male characteristics in vertebrates by binding to androgen receptors.This includes the activity of the primary male sex organs and development of male secondary sex characteristics. Androgens were first discovered in 1936. Androgens increase in both boys and girls during puberty.Androgens are also the original anabolic steroids and the precursor of all estrogens. The primary and most well-known androgen is testosterone.Dihydrotestosterone (DHT) and androstenedione are less known generally, but are of equal importance in male development.DHT in the embryo life causes differentiation of penis, scrotum and prostate. Later in life DHT contributes to balding, prostate growth and sebaceous gland activity. Although androgens are described as male sex hormones, both males and females have them to varying degrees, as is also true of estrogens. They are one of three types of sex hormones, the others being estrogens like estradiol and progestogens like progesterone.
Background: An androgen (from Greek andro meaning male human being) is any natural or synthetic compound, usually a steroid hormone, that stimulates or controls the development and maintenance of male characteristics in vertebrates by binding to androgen receptors.This includes the activity of the primary male sex organs and development of male secondary sex characteristics. Androgens were first discovered in 1936. Androgens increase in both boys and girls during puberty.Androgens are also the original anabolic steroids and the precursor of all estrogens. The primary and most well-known androgen is testosterone.Dihydrotestosterone (DHT) and androstenedione are less known generally, but are of equal importance in male development.DHT in the embryo life causes differentiation of penis, scrotum and prostate. Later in life DHT contributes to balding, prostate growth and sebaceous gland activity. Although androgens are described as male sex hormones, both males and females have them to varying degrees, as is also true of estrogens. They are one of three types of sex hormones, the others being estrogens like estradiol and progestogens like progesterone.
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Product Notes
The Rat Androgen (Catalog #AAA9712713) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712713 ELISA Kit recognizes Rat Androgen. It is sometimes possible for the material contained within the vial of "Androgen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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