IHC (Immunohistochemisry)
(B2M/Beta 2 Microglobulin Antibody-Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.)
Rabbit anti-Mouse, Human B2M/Beta 2 Microglobulin Polyclonal Antibody | anti-B2M antibody
B2M/Beta 2 Microglobulin Rabbit anti-Human Polyclonal Antibody
Reactivity
Mouse, Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Purity
Affinity purified
Synonyms
B2M/Beta 2 Microglobulin, Antibody; B2M/Beta 2 Microglobulin Rabbit anti-Human Polyclonal Antibody; PathPlus B2M/Beta 2 Microglobulin Antibody; B2M; Beta 2 Microglobulin; Beta-2-microglobulin; Beta-2-microglobin; anti-B2M antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human B2M/Beta 2 Microglobulin
Purity/Purification
Affinity purified
Form/Format
PBS, 50% glycerol, pH7.3
Concentration
3.06mg/ml (varies by lot)
Applicable Applications for anti-B2M antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence)
Target
Human B2M/Beta 2 Microglobulin
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human B2M (NP_004039.1). IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemisry)
(B2M/Beta 2 Microglobulin Antibody-Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.)
IHC (Immunohistochemisry)
(B2M/Beta 2 Microglobulin Antibody-Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.)
WB (Western Blot)
(B2M/Beta 2 Microglobulin Antibody-Western blot analysis of extracts of various cell lines, using B2M antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)
WB (Western Blot)
(B2M/Beta 2 Microglobulin Antibody-Western blot analysis of extracts of various cell lines, using B2M antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)
ICC (Immunocytochemistry)
(B2M/Beta 2 Microglobulin Antibody-Immunofluorescence analysis of MCF-7 cells using B2M antibody. Blue: DAPI for nuclear staining.)
ICC (Immunocytochemistry)
(B2M/Beta 2 Microglobulin Antibody-Immunofluorescence analysis of MCF-7 cells using B2M antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-B2M antibody
Beta 2 Microglobulin antibody is an unconjugated rabbit polyclonal antibody to Beta 2 Microglobulin (B2M) from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
B2M/Beta 2 Microglobulin is a serum protein associated with major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. It is required for proper cell surface expression of the complex. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In cancer, mutation, loss or deregulation of B2M is believed to be involved in aiding tumors in escaping the immune response and therapies. It is used as a marker in the identification of multiple myeloma and lymphoma, where it is regularly overexpressed. It has elevated levels in a number of other cancers, including colorectal, breast, gastric, lung, prostate and testicular cancer as well as malignant epithelial ovarian tumors. B2M has membranous and cytoplasmic positivity in most normal tissues, with elevated levels in immune cells and macrophages.
B2M/Beta 2 Microglobulin is a serum protein associated with major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. It is required for proper cell surface expression of the complex. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In cancer, mutation, loss or deregulation of B2M is believed to be involved in aiding tumors in escaping the immune response and therapies. It is used as a marker in the identification of multiple myeloma and lymphoma, where it is regularly overexpressed. It has elevated levels in a number of other cancers, including colorectal, breast, gastric, lung, prostate and testicular cancer as well as malignant epithelial ovarian tumors. B2M has membranous and cytoplasmic positivity in most normal tissues, with elevated levels in immune cells and macrophages.
References
J Transl Med. 2016; 14: 75, PMID: 26983758; Cancer Discov. 2017 Dec; 7(12): 1420 -1435, PMID: 29025772
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Protein Information
beta-2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin
UniProt Protein Name
Beta-2-microglobulin
UniProt Gene Name
B2M
UniProt Entry Name
B2MG_HUMAN
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Product Notes
The B2M b2m (Catalog #AAA162825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B2M/Beta 2 Microglobulin Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's B2M/Beta 2 Microglobulin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the B2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B2M/Beta 2 Microglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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