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Rat Acetylcholinesterase (ACHE) ELISA Kit | ACHE elisa kit

Rat Acetylcholinesterase (ACHE) ELISA Kit

Gene Names
ACHE; YT; ACEE; ARACHE; N-ACHE
Reactivity
Rat
Synonyms
Acetylcholinesterase (ACHE); Rat Acetylcholinesterase (ACHE) ELISA Kit; ACHE elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
72
Assay Type
Sandwich ELISA
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Range
25 nmol/L-400 nmol/L
Detection Method
Colorimetric
Limit of Detection
1.0 nmol/L
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ACHE elisa kit
Rat Acetylcholinesterase (ACHE) ELISA Kit employs a two-site sandwich ELISA to quantitate ACHE in samples. An antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Rat ACHE detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
43
NCBI Official Full Name
acetylcholinesterase, partial
NCBI Official Synonym Full Names
acetylcholinesterase (Cartwright blood group)
NCBI Official Symbol
ACHE
NCBI Official Synonym Symbols
YT; ACEE; ARACHE; N-ACHE
NCBI Protein Information
acetylcholinesterase
Protein Family

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Research Articles on ACHE

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Product Notes

The Rat ACHE (Catalog #AAA9711951) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711951 ELISA Kit recognizes Rat ACHE. It is sometimes possible for the material contained within the vial of "Acetylcholinesterase (ACHE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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