Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 3-mercaptopyruvate sulfurtransferase (MPST) Standard Curve.)
Rat 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit | MPST elisa kit
Rat 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit
Gene Names
Mpst; Mst
Reactivity
Rat
Synonyms
3-Mercaptopyruvate Sulfurtransferase (MPST); Rat 3-Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit; MPST; MGC24539; MST; TST2; 3-mercaptopyruvate sulfurtransferase; OTTHUMP00000197788; OTTHUMP00000197791; human liver rhodanese; MPST elisa kit
Reactivity
Rat
Specificity
Rat 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MPST. No significant cross-reactivity or interference between Rat MPST and analogues was observed.
Sequence Length
297
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 3-mercaptopyruvate sulfurtransferase (MPST) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 3-mercaptopyruvate sulfurtransferase (MPST) Standard Curve.)
Related Product Information for MPST elisa kit
Description: This Rat 3-mercaptopyruvate sulfurtransferase (MPST) ELISA Kit employs a two-site sandwich ELISA to quantitate MPST in samples. An antibody specific for MPST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: This protein encoded by MPST catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for MPST.
Background: This protein encoded by MPST catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for MPST.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
3-mercaptopyruvate sulfurtransferase
NCBI Official Synonym Full Names
mercaptopyruvate sulfurtransferase
NCBI Official Symbol
Mpst
NCBI Official Synonym Symbols
Mst
NCBI Protein Information
3-mercaptopyruvate sulfurtransferase
UniProt Protein Name
3-mercaptopyruvate sulfurtransferase
Protein Family
UniProt Gene Name
Mpst
UniProt Synonym Gene Names
MST
NCBI Description
catalyzes the formation of thiocyanide and pyruvate [RGD, Feb 2006]
Uniprot Description
MPST: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis.
Protein type: Amino Acid Metabolism - cysteine and methionine; EC 2.8.1.2; Mitochondrial; Transferase
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: cell junction; cytoplasm; cytosol; mitochondrial inner membrane; mitochondrion; neuron projection; synapse
Molecular Function: 3-mercaptopyruvate sulfurtransferase activity; identical protein binding; thiosulfate sulfurtransferase activity
Biological Process: kidney development; liver development; spinal cord development; transsulfuration
Research Articles on MPST
Similar Products
Product Notes
The Rat MPST mpst (Catalog #AAA9712240) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712240 ELISA Kit recognizes Rat MPST. It is sometimes possible for the material contained within the vial of "3-Mercaptopyruvate Sulfurtransferase (MPST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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